Cannon, who also directed the pilot, previously revealed his desire to incorporate the comic character into the new Fox TV series.
Confirming plans to introduce the character, Cannon said that he plans to reinvent Freeze's origins, reported Digital Spy.
"Without giving too much away - because I'm not allowed to - the great thing about working on a CSI procedural show for so long was we worked with so many experts - specialists in each field," he said.
"There's a lot of things I picked up along the way. My first conversation with showrunner Bruno Heller about the villains in this show is always that they need to be very credible and relatable and the science behind them needs to be real.
Real name Victor Fries, Mr Freeze was first introduced to comics in 1959. The character was the chief antagonist in 1997 film Batman & Robin - portrayed by Arnold Schwarzenegger.
The character also made three appearances in the '60s Batman television series - played first by George Sanders and later by Otto Preminger and Eli Wallach.
Batman villains confirmed to appear in Gotham include the Penguin (Robin Lord Taylor), Catwoman (Camren Bicondova), the Riddler (Cory Michael Smith) and Poison Ivy (Clare Foley).
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