Streets donned a deserted look during morning hours as private buses and state-run Kadamba Transport Corporation Ltd (KTCL) buses remained off the roads.
Various banks and insurance firms that have joined the stike call, also remained shut.
The situation is peaceful with no untoward incident reported in the state till now, a senior police official said.
"Towns of Calangute, Ponda and Bicholim remained totally shut. All shops and transport have remained closed in support of the strike," convenor, Goa Convention of Workers and All Goa Transport Operators Associations, Suhas Naik said.
"The strike is peaceful and we have appealed to people to maintain peace," Naik said.
All Goa Private Bus Owners Association president Sudip Tamankar said private buses have joined the strike and will not ply till evening.
Taxi service in the state was also affected as vehicles were withdrawn since last mid night.
"Taxis from airport and railway stations were withdrawn since last night. Not a single taxi was plying from morning," secretary, North Goa Tourist Taxi Owners Association, Vinayak Nanoskar said.
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