With 30 out of Scotland's 32 council areas having declared, the "No" side has an unbeatable lead of 1,877,252 votes to 1,512,688, official results confirmed today.
The winning total needed was 1,852,828 and nationally, the margin of victory is about 55 per cent to 45 per cent.
This margin of victory is some three points greater than that anticipated by the final opinion polls.
The vote is the culmination of a two-year campaign and talks will now begin on devolving more powers to Scotland, which had joined the United Kingdom in 1707.
But Edinburgh, the nation's capital, rejected independence by 194,638 to 123,927, while Aberdeen City voted "No" by a margin of more than 20,000 votes.
There have also been big wins for the pro-UK campaign in many other areas.
British Prime Minister David Cameron said: "I've spoken to Alistair Darling (head of the pro-UK Better Together campaign) - and congratulated him on a well-fought campaign."
He will make official statement on Scotland's decision in a live televised address following the final result.
"There is an appetite of change in Scotland, this country has changed forever," he added.
After votes have been tallied, the chief counting officer, Mary Pitcaithly, in Edinburgh, is set to officially declare the result followed by a statement by Queen Elizabeth II.
The people of Scotland had been queueing up to register their vote in the historic referendum yesterday to decide their future as part an independent country or the UK.
They had a simple "Yes" or "No" choice to the question: "Should Scotland be an independent country?"
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