An LBG spokesman said the ban was across its Lloyd Bank, Bank of Scotland, Halifax and MBNA branded credit cards.
In a brief statement, he said LBG does "not accept credit card transactions involving the purchase of cryptocurrencies".
Over the past few days, US lenders Bank of American, Citigroup and JPMorgan each introduced the same ban.
There is a concern that customers who bought bitcoin late last year when crytocurrencies in general surged in value have been left with big losses following massive declines in recent weeks.
It comes as China plans to stamp out all remaining cryptocurrency trading in the country by blocking access to overseas-based websites and removing related applications from app stores.
The international value of bitcoin and other cryptocurrencies have plunged this year amid fears of a crackdown in Asia and concerns that many currencies' rapid rise in value last year could reflect an bubble.
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