"When the United Nations consistently fails in its duty to act collectively, there are times in the life of states that we are compelled to take our own action," US Ambassador Nikki Haley said.
The warning came during an emergency meeting of the UN Security Council called by France and Britain after the attack in the early hours on Tuesday on a rebel-held town in Idlib province.
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"How many more children have to die before Russia cares?" she asked.
"If Russia has the influence in Syria that it claims to have, we need to see them use it," she said. "We need to see them put an end to these horrific acts."
At least 72 people, among them 20 children, were killed in the strike on Khan Sheikhun, and dozens more were left gasping for air, convulsing, and foaming at the mouth, doctors said.
It is thought to be the worst chemical weapons attack in Syria since 2013, when sarin gas was used.
"If we are not prepared to act, then this council will keep meeting, month after month to express outrage at the continuing use of chemical weapons and it will not end," Haley said.
"We will see more conflict in Syria. We will see more pictures that we can never unsee."
Britain, France and the United States have presented a draft resolution demanding a full investigation of the attack, but Russia said the text was "categorically unacceptable."
The draft resolution backs a probe by the Organisation of the Prohibition of Chemical Weapons (OPCW) and demands that Syria cooperate to provide information on its military operations on the day of the assault.
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