The New York-based rights watchdog accused rebels of using "children as young as 15 to fight in battles" as they try to overthrow President Bashar al-Assad in a bloody conflict that has lasted more than three years.
Some rebel groups recruit teenagers "under the guise of offering education," HRW said in the report published today.
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The HRW report was based on the experiences of 25 child soldiers - some of whom are still fighting - who were involved in ISIL, the Free Syrian Army (FSA), the Islamic Front, Syria's Al-Qaeda affiliate the Al-Nusra Front as well as Kurdish forces.
The children interviewed by HRW said they took part in combat, worked as snipers, manned checkpoints, spied, cared for the wounded, or carried munitions or other supplies to the front line.
Many said they had joined up to follow friends or family, while others said they enlisted after taking part in the peaceful demonstrations calling for political change that sparked the conflict in March 2011.
"Syrian armed groups shouldn't prey on vulnerable children - who have seen their relatives killed, schools shelled, and communities destroyed - by enlisting them in their forces," HRW's Priyanka Motaparthy said.
"The horrors of Syria's armed conflict are only made worse by throwing children into the front lines."
The number of child soldiers fighting in the Syrian conflict is unknown, but the Violations Documentation Centre, a organisation close to the opposition, reported that 194 "non-civilian" children have been killed since September 2011.
HRW also urged countries supporting the uprising against Assad to press for an end to child recruitment.
"Governments supporting armed groups in Syria need to press these forces to end child recruitment and use of children in combat," Motaparthy said.
"Anyone providing funding for sending children to war could be complicit in war crimes."
A number said they had joined opposition groups after being personally mistreated by Assad's security forces.
Some of the children interviewed by HRW said they had joined opposition groups after being mistreated by Assad's security forces.
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