Canberra temporarily halted flights on Tuesday after a spike in tensions between the US and Russia, which warned it would track coalition aircraft in Syria as potential "targets".
Moscow also halted a military hotline with Washington over the incident, intended to prevent confrontations in Syria's crowded air space.
Australia defence ministry said in a statement the suspension was "a precautionary measure to allow the coalition to assess the operational risk".
"The suspension has since been lifted," it added.
A top US general said the country would work to relaunch the "deconfliction" hotline established in 2015, after Russia said Washington had failed to use the line -- a vital incident-prevention tool -- before targeting the plane near Raqa.
Australia is part of the coalition fighting the Islamic State group in Iraq and in late 2015 extended air operations into Syria, with a total of 780 defence personnel based in the Middle East.
Australia's Air Task Group consists of 300 personnel, six F/A-18 Hornets, an E-7A Wedgetail Airborne Early Warning and Control aircraft, and a KC-30A Multi-Role Tanker and Transport plane.
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