Coulter-Nile, 30, has a history of spinal stress fractures and the latest setback appears to end his chances of being a back-up to Australia's pace trio Mitchell Starc, Josh Hazlewood and Pat Cummins against England.
"Nathan experienced some pain in his back following the two-day tour match against England last week," CA's sports medicine manager Alex Kountouris said.
"Subsequent scans have revealed an early stage aggravation of his old stress fracture.
Kountouris said Coulter-Nile will have further scans over the next month to determine when he can return to bowling. The five-Test Ashes series starts on November 23.
Coulter-Nile has yet to play a Test match, but has made 21 one-day and 19 T20 international appearance for Australia.
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