With 66 votes for and three abstentions, the parliament in Namur, the main city of southern French-speaking Wallonia, adopted a ban that will generally take effect in June 2018, though ritual slaughter will be exempted until September 2019.
The ban on slaughter without first stunning or anaesthetising the animals does not apply to imports of kosher or halal meat into the Wallonia region.
Animal rights activists from groups like GAIA pushed for the ban which violates both kosher and halal ritual slaughter where the animals are required to be conscious when their throats are slit.
Brussels-based European Jewish Association spokesman Alex Benjamin told AFP just before the vote that the Wallon parliament's adoption of the legislation plays "into certain hands" at a time of a rising populist right.
"Either unwittingly or wittingly, it has a negative effect. It sends a signal to the Jewish population here... that we don't really respect you or your practices."
He said there is no scientific evidence that kosher slaughter is less humane than other practices.
"There's no nice way of taking an animal's life," Benjamin said.
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