Sheen filed the suit on Friday in Los Angeles Superior Court against the tabloid, its parent company American Media, Inc.,Dylan Howard, the tabloid's chief content office and Dominick Brascia, who told the publication the "Two and Half Men" star raped Haim, reported Variety.
In his lawsuit, Sheen called the story part of an "egregious, hurtful and disgusting campaign of defamations."
"With calculated malice, the National Enquirer has concluded that it can make money by running false and salacious stories claiming that Mr Sheen is a sexual molester that preys on young pubescent boys," the lawsuit states.
American Media Inc., the parent company of the Enquirer, said it would defend the suit.
"We look forward to litigating against Charlie Sheen, and cant wait to expose his depravities in a court of law," the company said in a statement.
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