Rajendran was being "relieved" as the district secretary of Ariyalur, Dhinakaran said in a statement, while continuing to effect changes in the party ranks.
He appointed P Muthiayan in Rajendran's place.
On Thursday, Rajendran had petitioned Assembly Speaker P Dhanapal for the disqualification of the 19 pro-Dhinakaran MLAs who had revolted against Chief Minister K Palaniswami.
The Dhinakaran-loyalist MLAs had met Governor Ch Vidyasagar Rao seeking removal of Palaniswami as the chief minister.
Citing reports, Rajendran had said the MLAs told Rao they were "withdrawing" support to Palaniswami even as there was no change in the party's February 14 unanimous decision to elect him as AIADMK Legislature Party Leader.
The legislators had also addressed the media, which was an "anti-party activity" and the act implied "voluntary relinquishing of membership", Rajendran had said.
They were therefore liable to be disqualified under the 10th Schedule of the Constitution, he had said in his petition to the Speaker, besides quoting from a Tamil Nadu anti- defection law.
Villupuram (South) functionary and MLA R Kumaraguru was being replaced by K G P Gnanamoorthy, Dhinakaran said.
He also removed Lok Sabha MP P R Senthilnathan as the party's Sivaganga district secretary. K K Umadevan has been declared the new district secretary.
All the changes were being made with the "approval" of party chief V K Sasikala, Dhinakaran said.
Dhinakaran, who is involved in a tussle for power with Palaniswami, has been reshuffling the party ranks in the past few days. He has removed, among others, five ministers, from party posts and replaced them with his supporters.
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