The DMK Legal Wing has served the notices to State's Transport Minister Senthil Balaji, Chief Secretary Sheela Balaakrishnan and Managing Director of Metropolitan Transport Corporation among others.
The M Karunanidhi led party has also petitioned the Election Commission to derecognise AIADMK for violating model code of conduct in the Yercaud Assemby bypoll.
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The complaint stated that AIADMK with calculative and scientific motive has painted its "two leaves" symbol on 50 mini-buss launched on October 23 and also on some 610 buses.
The action amounted to misuse and abusing the official position as public servant and also a criminal misconduct, it charged.
The complaint besides urging Jayalalithaa to remove the advertisements has also appealed to CAG to look into the issue.
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