Among the 230 activists was leading campaigner Ahmed Douma, who is already serving a three-year-sentence for breaking a draconian law regulating protests.
Douma rose to prominence during the 2011 uprising and was also a key protest leader against Mubarak's successor Mohammed Morsi, who is currently in prison over charges of killing peaceful protesters, espionage and escaping from prison.
A life-in-prison verdict carries a sentence of 25 years in jail according to the Egyptian penal code.
Some 39 other defendants, who are minors, were hit with 10 years in prison in the same case, Alahram reported.
It is the heaviest sentence yet against non-Islamist activists who spearheaded the mass protests. The ruling can be appealed.
In 2011, clashes erupted near Tahrir Square, the focal point of the revolt, when a sit-in was forcibly dispersed by security personnel, sparking clashes between protesters and army and police forces.
During the protests, a fire gutted parts of a library housing rare books and manuscripts in the Egyptian capital.
Since the ouster of Morsi in 2013, the Egyptian government has been also cracking down on the outlawed Muslim Brotherhood and its supporters.
The government has declared the Brotherhood a terrorist organisation.
Earlier this week, 183 Morsi supporters were sentenced to death by a court over killing of 15 police officials in an attack in 2013.