US lawmakers are scheduled to consider the sanctions package as early as Tuesday, and the bill could be sent to President Donald Trump before Congress breaks for the August recess.
The measures are aimed at punishing Moscow for meddling in the presidential election and its military aggression in Ukraine and Syria.
EU member states Germany and Austria have criticized the penalties, saying they could affect European businesses involved in piping in Russian natural gas.
European Commission spokesman Margaritis Schinas said Monday that the EU's executive arm is "following this process with some concern regarding the European Union's energy independence and our energy security interests."
He would not speculate on any possible EU retaliation should European businesses be hurt by US sanctions.
"We in the European Union will have interests in this discussion and we expect this interest to be addressed by the ongoing pre-legislative process" in the United States, Schinas said.
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