The assault targeted IS positions south of the town of Ras al-Ain in Hasakeh province, the Britain-based monitoring group added.
Ras al-Ain was the scene of fierce fighting last year before Kurdish forces ousted the jihadists from the border town.
The Observatory, which relies on activists and medics on the ground for its information, also reported clashes at the jihadist-besieged border town of Kobane, some 100 kilometres (60 miles) away.
IS fighters, who already control swathes of Syria and neighbouring Iraq, launched an assault on the town on September 16.
The Observatory also said IS jihadists had beheaded four men in public in the central province of Homs after accusing them of "insulting God".
Syria's conflict began in March 2011 with protests against the regime of President Bashar al-Assad, escalating into armed conflict when his forces cracked down hard on dissent.
Nearly four years of civil war ravaging the country have killed an estimated 200,000 people and also displaced half of the population.
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