The fishermen from Kottaipattinam in Pudukottai district have been taken to Kangesanthurai port, police said.
Their boat was also seized for allegedly intruding into Lankan territory and poaching in their zone, they said.
On Friday last, eight fishermen from Nagapattinam district were arrested for allegedly fishing off Neduntheevu, while four fishermen from Jagadapattinam in Pudukottai district were arrested on July 17.
On July 13, seven fishermen from Mandapam near here were arrested for allegedly fishing in Lankan waters.
The state government has maintained that restoration of Katchatheevu, an islet ceded to Sri Lanka in the 1970s, was the "only solution" to the vexed fishermen's issue.
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