Anwar, 69, began serving a five-year jail term last year for sodomising a male aide. He had denied the charges.
He had filed 35 grounds in his petition of appeal asking the Federal Court to overturn the Court of Appeal's ruling.
A panel of five judges ruled unanimously that Anwar's application for a review of his 2014 conviction, his final legal option for an acquittal, was without merit.
Chief Judge of Malaya, Zulkefli Ahmad Makinudin said the decision was unanimous.
"It is not end of the road," he said.
Human Rights Watch called the decision by the Federal Court a "real tragedy for justice in Malaysia".
Anwar was heir-apparent to leadership of the ruling United Malays National Organisation (UMNO) in the late 1990s until his ouster in a power battle with then-leader Mahathir Mohamad.
He was jailed in 1998 for six years on sodomy and corruption charges that he also called politically motivated.
Anwar, a former deputy premier, was charged in 2008, months after his opposition scored unprecedented gains in general elections against the Barisan Nasional coalition, which has ruled the country for over five decades.
The charges were slapped on him after he had fallout with Mahathir, Malaysia's prime minister for 22 years.
He was freed in 2004 after spending six years in prison after a court overturned the sodomy conviction.
He later became a firebrand opposition leader and led his opposition alliance to an unexpected victory at the hustings in 2008 while the ruling coalition of Barisan Nasional won by a thin margin to retain power.
Disclaimer: No Business Standard Journalist was involved in creation of this content
You’ve reached your limit of {{free_limit}} free articles this month.
Subscribe now for unlimited access.
Already subscribed? Log in
Subscribe to read the full story →
Smart Quarterly
₹900
3 Months
₹300/Month
Smart Essential
₹2,700
1 Year
₹225/Month
Super Saver
₹3,900
2 Years
₹162/Month
Renews automatically, cancel anytime
Here’s what’s included in our digital subscription plans
Exclusive premium stories online
Over 30 premium stories daily, handpicked by our editors


Complimentary Access to The New York Times
News, Games, Cooking, Audio, Wirecutter & The Athletic
Business Standard Epaper
Digital replica of our daily newspaper — with options to read, save, and share


Curated Newsletters
Insights on markets, finance, politics, tech, and more delivered to your inbox
Market Analysis & Investment Insights
In-depth market analysis & insights with access to The Smart Investor


Archives
Repository of articles and publications dating back to 1997
Ad-free Reading
Uninterrupted reading experience with no advertisements


Seamless Access Across All Devices
Access Business Standard across devices — mobile, tablet, or PC, via web or app
