An Australian naval aircraft picked up an underwater signal in the same area where searchers in ships previously detected sounds consistent with a plane's black box.
The development came on the 33rd day of the search for the Malaysia Airlines Flight MH370 - that vanished mysteriously from radar screens on March 8 with 239 people on board, including five Indians.
"The acoustic data will require further analysis overnight but shows potential of being from a man-made source," he said.
Houston yesterday expressed optimism that search teams will "find the aircraft or what's left of the aircraft in the not too distant future".
"I believe we are searching in the right area but we need to visually identify wreckage before we can confirm with certainty that this is the final resting place of MH370," Houston said.
The batteries of the black box flight recorders have a life of about 30 days, meaning they either might have been or are on the verge of being drained out.
Stored in a plane's tail, they are designed to begin sending off distinct, high-pitched pings or signals as soon as they come in contact with water.
The Ocean Shield, bearing a special US Navy "towed pinger locator", had picked up two fresh signals on Tuesday that matched a pair of signals detected over the weekend consistent with a plane's black box.
Up to 10 military aircraft, four civil aircraft and 13 ships were assist in today's search for the plane that authorities believe crashed in the Indian Ocean.
The three vessels - the Ocean Shield to the north, and the British HMS Echo and Chinese Haixun 01 to the south -- were focussing underwater.
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