"Our country has just been attacked once more by terrorist groups, an assault which sadly has resulted in a large number of casualties," he said at a regional meeting in the capital Niamey.
Yesterday, a US-Nigerien patrol was ambushed in southwestern Niger near the border with Mali, the Pentagon and Nigerien officials said.
Three US soldiers were killed and two were wounded, the US Africa Command said in a statement from its headquarters in Germany.
There was a fourth fatality from another nation, according to the US statement, which did not give further details.
Issoufou spoke at the opening of a forum of first ladies of the West African regional group Ecowas, which was dedicated to obstetric fistula, a leading source of child birth injuries.
He called for a minute's silence "to the memory of our soldiers who have fallen on the field of honour" and to the memory of "all victims of terrorism."
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