That's more than 1,500 years earlier than previously believed, scientists say.
"This is a big deal," said Jessi Halligan, one of the study's authors and an assistant professor of anthropology at Florida State University.
"It's pretty exciting. We thought we knew the answers to how and when we got here, but now the story is changing."
The discovery on the Aucilla River was reported on Friday by the journal Science Advances.
There, divers excavated artifacts such as butchered bones of extinct animals, a mastodon tusk and a biface, which is a knife fragment with sharp edges.
"At Page-Ladson, hunter-gatherers, possibly accompanied by dogs, butchered or scavenged a mastodon carcass at the sinkhole's edge next to a small pond at ~14,550," the authors said in Science Advances.
What was once a pond was buried beneath the murky waters for a series of reasons, including centuries of civilization, rising sea levels and layers of sediment.
The scientists used radiocarbon dating techniques to find out how old the artifacts are, CNN reported.
Until that point, researchers had believed the Clovis people were among the first inhabitants of the Americas about 13,000 years ago, according to the study.
Page-Ladson is the first pre-Clovis site documented in the southeastern part of North America, it said.
"The new discoveries at Page-Ladson show that people were living in the Gulf Coast area much earlier than believed," said Michael R. Waters, director of Texas A&M's center for the study of the first Americans. Waters was one of the study's lead authors.
Halligan and her colleagues returned to the site in 2012 and expanded on the previous research and archaeological finds. In one of the instances, a mastodon tusk recovered earlier had deep grooves. They concluded the grooves were made by humans during the tusk's extraction.
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