Messi was involved in all three goals as he beat Gianluigi Buffon for the first time in his career to open the floodgates just before half-time late last night.
Ivan Rakitic added a second after Messi's run and cross opened up the Juventus defence before the Argentine rounded off the scoring by drilling home his second of the evening 19 minutes from time.
Victory puts Barca on top of Group D alongside Sporting Lisbon, who were 3-2 winners at Olympiakos.
However, Ousmane Dembele impressed on his full debut following a 105 million-euro move from Borussia Dortmund, as Barca proved as long as they have Messi there is life after Neymar.
Juventus showed even more upheaval since the sides last met in last season's quarter-finals with six changes from their trip to the Camp Nou just five months ago.
The Italian champions progressed comprehensively 3-0 on aggregate back then and threatened to expose Barca again early on with Marc-Andre ter Stegen forced into three early saves to deny Mattia de Sciglio, Paulo Dybala and Miralem Pjanic.
Their best effort before opening the scoring came when a Messi free-kick rebounded back into Luis Suarez's path and his fierce effort was turned over by Buffon.
Dembele was involved in the move as Messi broke his personal duck against Buffon in the final minute of the first half.
Messi picked up the Frenchman's pass before exchanging a one-two with Suarez and firing with unerring accuracy just inside the far post.
Juventus had a big chance to respond right at the start of the second half, but Dybala couldn't match his international teammate's finish and he lifted his shot over the bar.
Yet, Messi was also fortunate to escape his first ever red card at club level moments later as he was booked by Slovenian referee Tomaz Klancnik for his attempts to get a Juventus player cautioned.
Aided by his teammates, though, Messi continued his protests and could easily have been shown a second yellow by a more stringent official.
His clash with the referee only seemed to inspire Messi to greater heights as he drove through the Juventus defence once more and, when his low cross was only partially cleared by Stefano Sturaro, Rakitic slotted home the rebound.
However, the veteran goalkeeper was helpless once more 21 minutes from time when Messi cut inside onto his left foot and buried the ball into the bottom corner for his eighth goal of the season already.
Disclaimer: No Business Standard Journalist was involved in creation of this content
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