The pro-Western leader said the weekend capture of two purported Russian special forces members proved that the separatist uprising in the industrial east of Ukraine was a guise for a Moscow-orchestrated campaign aimed at breaking up the ex-Soviet state.
"Can I be absolutely clear with you this is not a fight with Russian-backed separatists, this is a real war with Russia," the 49-year-old Ukrainian leader told the BBC.
"The fact that we captured... Russian regular special forces soldiers (is) strong evidence of that."
The men testified during a taped interrogation that they entered the warzone nearly two months ago as part of a 200-strong reconnaissance unit from the Russian army's Main Intelligence Directorate (GRU).
A Ukrainian Security Service spokesman said the suspects have been charged with involvement in "terrorist activity" and given a chance to phone their relatives back home.
Moscow acknowledges the presence of Russian "volunteers" and off-duty servicemen in Ukraine but rejects charges that they are there under orders from President Vladimir Putin's generals.
Kiev's detention and display before world media yesterday of the two soldiers captured in Lugansk has outraged the Kremlin and threatened to cement Putin's resolve to stamp his own solution on the crisis that would keep Kiev within Moscow's orbit for years.
Putin's spokesman Dmitry Peskov said flatly that the "Kremlin does not agree" with what the Ukrainian leader told the BBC.
"First of all, one has to understand that unfortunately, Kiev is waging war against its own citizens," Russian news agencies quoted Peskov as saying.
The United Nations believes the more-than-year-long conflict has claimed at least 6,250 lives and driven more than a million people from their homes.
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