The rock star said property tycoon Trump, the presumptive Republican nominee in the US presidential elections, was using the song without permission.
May, 68, said he had received an "avalanche of complaints" about Trump using the anthem during an appearance on US television on Tuesday.
"Permission to use the track was neither sought nor given. We are taking advice on what steps we can take to ensure this use does not continue," May wrote on his website.
"Our music embodies our own dreams and beliefs, but it is for all who care to listen and enjoy."
"We Are The Champions" was written by the group's late lead singer Freddie Mercury in 1977 and is an enduring celebratory anthem heard at sports finals.
Queen join a long list of artists who have refused to allow Trump to use their music, including The Rolling Stones, Neil Young, R. E. M. And Adele.
Queen are currently on a tour of European festivals with US singer Adam Lambert.
You’ve reached your limit of {{free_limit}} free articles this month.
Subscribe now for unlimited access.
Already subscribed? Log in
Subscribe to read the full story →
Smart Quarterly
₹900
3 Months
₹300/Month
Smart Essential
₹2,700
1 Year
₹225/Month
Super Saver
₹3,900
2 Years
₹162/Month
Renews automatically, cancel anytime
Here’s what’s included in our digital subscription plans
Exclusive premium stories online
Over 30 premium stories daily, handpicked by our editors


Complimentary Access to The New York Times
News, Games, Cooking, Audio, Wirecutter & The Athletic
Business Standard Epaper
Digital replica of our daily newspaper — with options to read, save, and share


Curated Newsletters
Insights on markets, finance, politics, tech, and more delivered to your inbox
Market Analysis & Investment Insights
In-depth market analysis & insights with access to The Smart Investor


Archives
Repository of articles and publications dating back to 1997
Ad-free Reading
Uninterrupted reading experience with no advertisements


Seamless Access Across All Devices
Access Business Standard across devices — mobile, tablet, or PC, via web or app
