Dealers said early losses in domestic equity markets also weighed on the rupee.
Yesterday, the rupee had staged an incredible comeback after its three-session listless trade and gained 26 paise to end at fresh two-and-a-half month high of 64.11 a dollar.
The dollar weakened globally after the US Federal Reserve indicated that it would maintain a slow pace of monetary tightening.
The benchmark BSE Sensex fell 189.67 points, or 0.58 per cent, to 32,193.63 in early trade today.
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