In a Twitter posting, the tournament said top halves of brackets on both the men's and women's draws would play matches on the first day of competition.
That means three-time defending champion Williams, trying to complete the first calendar-year Grand Slam since Steffi Graf in 1988, will meet Russia's Vitalia Diatchenko on day one, likely in a feature match on Arthur Ashe Stadium and quite possibly in the night session opener.
Williams, coming off her fourth consecutive major title last month at Wimbledon, seeks her 22nd career Grand Slam singles title, which would match Graf for the all-time Open Era record and move her two shy of Margaret Court's all-time record of 24.
The other top half quarter of the men's bracket will see Japanese fourth seed Kei Nishikori, last year's US Open runner-up, face France's Benoit Paire in his first match while defending US Open men's champion Marin Cilic, a ninth seed from Croatia, opens against Argentina's Guido Pella.
The tricky quarter-bracket with Williams also features her older sister Venus, a seven-time Grand Slam champion, meeting Puerto Rico's Monica Puig in round one.
No exact times have been announced for any matches on Monday.
In past years, the US Open schedule has seen the men play the first round over three days and set up a "Super Saturday" with the men's semi-finals and women's final.
But that scheduling was scrapped in favor of making sure the men and women each have an off day ahead of the championship match, which did not happen in the "Super Saturday" format.
Kyrgios sparked outrage after comments to Wawrinka during a match about his girlfriend having possibly been unfaithful.
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