The separatists chose October 1, the anniversary of the official reunification of the anglophone and francophone parts of Cameroon, to declare independence for "Ambazonia", the name of the state they want to create.
Since November, the anglophone minority has been protesting against perceived discrimination.
The government deployed security forces at the weekend in English-speaking regions, notably Buea in the southwest and Bamenda, the main town in the northwest and a hub of anglophone agitation.
Several people were admitted to hospital in Bamenda Sunday after clashes between demonstrators and police, according to a medical source.
The "security forces had to resort to tear gas and sometimes to shots to disperse the protesters", the source said by telephone.
Bamenda residents contacted by AFP reported "shooting" by the security forces without giving further details.
One of the leaders of the opposition Social Democratic Front (SDF), Joshua Osih, told AFP the security forces were "firing real bullets at the protesters" but stressed that he was not a supporter of the secessionist movement.
Also in Kumbo, three prison inmates were shot and killed trying to escape while security forces were mobilised for the deployments in anglophone regions, a source close to regional authorities said.
A young man was shot dead by security forces on Saturday in the southwest town of Kumba, known as a rebellious city since the start of the protests, sparking clashes between security forces and the local population.
"They fired at him during a security operation," a nurse who requested anonymity told AFP. The incident was confirmed by a security source and several local residents contacted by phone.
The European Union called on all sides to be responsible and "respect the rule of law and avoid any act of violence.
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