Qantas said a "number of passengers travelling in the same group on QF28 (between) Santiago and Sydney became unwell with a gastro intestinal illness" on the 14-hour journey to Australia.
Media reports said between 20 and 30 passengers were vomiting as they disembarked in Sydney and were met by a fleet of ambulances.
"It is believed the illness was contracted before boarding the aircraft and symptoms became evident during the flight," Qantas said in a statement.
As a precaution, Qantas said all other passengers on the flight were advised to monitor their health closely over the next 24-48 hours.
Health authorities said they had been advised that "26 passengers returning on a Qantas flight from Santiago, Chile, developed vomiting and diarrhoea on the plane, consistent with norovirus infection, a common cause of outbreaks of gastroenteritis in Australia and elsewhere".
"New South Wales Health has provided Qantas with a letter and factsheet about viral gastro to distribute to all departing passengers, as well as advice for cleaning and cabin staff," the state's health office said in a statement.
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