"After coalition air strikes, the main water line was ruptured and water was cut to all of Raqa city," said Hamoud al-Mousa of the Raqa is Being Slaughtered Silently activist collective.
His group said the overnight strikes hit several bridges in the north of Raqa, including one known as the Old Bridge.
Mousa said a pipeline running along the Old Bridge into Raqa -- the only way residents could get water -- had been destroyed.
Raqa, 90 kilometres south of the Turkish border, is IS's de facto capital in Syria and remains home to more than 300,000 people.
The city and surrounding province have been battered by air strikes carried out by the US-led coalition fighting IS and by Russia, a key ally of Syria's government.
The coalition raids are backing an alliance of Arab and Kurdish fighters who launched a major offensive against Raqa in November.
The Syrian Observatory for Human Rights confirmed water to Raqa had been cut after "probable" coalition raids.
The Observatory, which relies on a wide network of sources inside Syria for its information, says it determines whose planes carry out raids according to type, location, flight patterns and munitions used.
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