The High Negotiations Committee (HNC) said recent attacks near Damascus, Homs city, and elsewhere were "obstructing the efforts aimed at a political transition in Syria".
"It is a bloody message from a criminal regime just a few days ahead of political negotiations in Geneva that demonstrates its rejection of any political solution," the HNC said in an online statement.
Armed opposition groups went even further, accusing the regime of eliminating any shot at a peaceful resolution to Syria's war.
At least 16 people were killed on Saturday when government forces shelled a funeral near Damascus, and three civilians were killed in air strikes on the last rebel-held district of Homs city, according to the Syrian Observatory for Human Rights.
And on Sunday, seven civilians were killed in air strikes on a town in southern Syria, while an exchange of shelling in Daraa city killed a nurse and a young girl, the monitor said.
Formed in December 2015, the HNC has risen to prominence as the leading umbrella group for Syrian opposition factions.
Sabra will replace Mohamed Alloush of the Army of Islam rebel group, which said it would participate in the delegation in an advisory capacity.
Alloush has twice headed the rebel delegation to talks in Kazakhstan, where opposition backer Turkey and regime allies Russia and Iran organised parallel negotiations.
Meetings in Astana have focused on confidence-building measures and reinforcing a frail, seven-week truce deal also brokered by Ankara and Moscow.
His role in Syria's future remains the primary sticking point, with the opposition insisting that he leave at the beginning of any transition period.
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