Rob Ford will instead seek a seat on the City Council, after a nephew withdrew his candidacy.
"My heart is heavy when I tell you that I'm unable to continue my campaign for re-election as your mayor," Ford said in a statement. "I have asked Doug to run to become the next Mayor of Toronto, because we need him. We cannot go backwards."
Ford's decision came two days after he was hospitalised and the tumor was discovered. Biopsy results won't be back for a week and a definitive diagnosis is pending.
"As many of you know I've been dealing with a serious medical issue, the details of which are unknown. But I know that with the love and support of my family, I will get through this," Ford said in a statement.
The international spotlight first fell on Ford in May 2013, when Toronto Star and the US website Gawker reported the existence of a video apparently showing the mayor inhaling from a crack pipe. He denied the existence of the video for months until police announced they had obtained it.
After reports emerged this year of a second video showing him apparently smoking crack, Ford decided to enter rehab. But he refused to quit his job or abandon his bid for re-election.
Ford has been the subject of an ongoing police investigation but has not been charged.
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