US officials have often accused Pakistan of helping the militants, a charge Islamabad denies.
"The president has also made clear that he, that we need to see a change in- in behaviour of those in the region, which includes- those who are providing safe haven and support bases for the Taliban, Haqqani Network and others," the National Security Advisor, McMaster, was quoted by MSNBC as saying.
He said that Trump is making clear that the US will no longer "tolerate" any support for the Taliban or related groups.
"Of course, you know, a very paradoxical situation, right, where Pakistan is taking great losses. They have fought very hard against these groups, but they've done so really only selectively," he said.
Pakistan's two neighbours -- both India and Afghanistan - have accused it of being selective in its war against terrorism.
He also defended Trump's strategy on winning the war in Afghanistan by giving unrestricted powers to the US military based in the war-torn country.
"The president has said that, "He does not want to place restrictions on the military that undermine our ability to win battles in combat".
"He has lifted those restrictions, and you are beginning to see the payoff of that as well," the top US national security advisor said.
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