Ban complained in a report to the UN Security Council of 67 cases of "harassment, threats, physical assault, arrest and detention of United Nations staff" and seizure of UN vehicles between May 7 and November 5.
The US government took up the complaints in an official approach to South Sudanese diplomats in New York yesterday, diplomatic sources said. The UN Security Council is to discuss South Sudan on Monday.
Ban said most of the cases involved the Sudan People's Liberation Army (SPLA), which has already been involved in shooting down a UN helicopter, and the national police.
Diplomats said the case involved a convoy of South Sudan's President Salva Kiir.
From October 25 to November 3, another five cases of "harassment and extortion" of UN staff by South Sudanese security personnel were reported in Juba, Ban added.
Outside Juba, about 50 SPLA soldiers tried to force their way onto a helicopter belonging to the UN Mission in South Sudan (UNMISS) in troubled Jonglei state on October 26, the report said.
The UN normally has a status of forces agreement with countries where it operates.
Ban said: "Continuing violations of the status of forces agreement between the United Nations and South Sudan by elements of the security sector remain a serious concern.
"I call upon the government to urgently take steps to prevent any recurrences and bring to account those responsible for violations, including the incident in Juba on October 19," the UN leader said.
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