The Supreme Security Committee said a Thursday air strike that killed 17 people, mostly civilians, near Rada in the central province of Bayda, had targeted Al-Qaeda.
"If the government fails to stop American planes from... bombing the people of Yemen, then it has no rule over us," tribal chief Ahmad al-Salmani told AFP from Rada.
He spoke as hundreds of armed men from the large Qayfah tribe blocked the Rada-Sanaa road linking several southern areas to the capital, witnesses said.
The mediation committee was said to have travelled today to Sanaa to present the tribe's demands, a local official said.
"The first demand is an end to strikes. They also want financial and moral compensation," the official said.
The committee later met the regional military command in Rada, another official told AFP.
"They are discussing solutions to be presented to the families of civilian casualties," the official said, adding that tribal arbitration, which would stipulate compensation, was the probable outcome.
The security committee headed by President Abdrabuh Mansur Hadi said Thursday's strike targeted "a car that belonged to one of the leaders of Al-Qaeda".
A statement carried by the state news agency Saba did not give a death toll for the strike, mention any civilian casualties or admit that it was a US drone attack.
Security sources and witnesses had said that the attack by two missiles hit a wedding convoy, killing mostly civilians.
Two of the dead whose names were released -- Saleh al-Tays and Abdullah al-Tays -- had figured on past government lists of wanted Al-Qaeda suspects.