The G7 meeting, attended in person by U.S. Secretary of State Antony Blinken and his counterparts from France, Italy, Germany, Japan and Canada, comes amid international concern that Russia could invade Ukraine. Russia denies planning any attack.
Any incursion by Russia "would be a strategic mistake for which there would be serious consequences," the foreign office added.
"We need to defend ourselves against the growing threats from hostile actors and we need to come together strongly to stand up to aggressors who are seeking to limit the bounds of freedom and democracy," Truss told foreign ministers at the start of the meeting.
"To do this, we need to have a stronger united voice" Ukraine is at the centre of a crisis in East-West relations as it accuses Russia of massing tens of thousands of troops in preparation for a possible large-scale military offensive.
Ministers arrived at the Museum of Liverpool to a brass band playing Christmas carols, before convening the first formal meeting session which will look at geopolitical issues including nuclear talks with Iran and the military buildup in Iran.
"What the G7 meeting this weekend .. is about, is about a show of unity between like-minded major economies, that we are going to absolutely be strong in our stance against aggression, against aggression with respect to Ukraine," Truss told reporters ahead of the talks.
AGE OF INTROSPECTION
Britain, as current chair of the G7, is calling for its members to be more strident in their defence of what it calls "the free world".
The G7 meeting is also expected to result in a joint call for Iran to moderate its nuclear programme and grasp the opportunity of ongoing talks in Vienna to revive a multilateral agreement on its nuclear development.
Both Truss and Blinken stressed the need for Iran to engage in the talks, according to the foreign office statement.
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