The move by the agency came months after Hemraj's wife had in December last year approached the top court against the Allahabad High Court verdict of October 12 last year acquitting the couple.
The CBI filed an appeal challenging the high court order acquitting the Talwar couple, CBI's spokesperson said.
The high court had freed them of the charges on the ground that they could not be held guilty on the basis of the evidence on record.
Before the high court's order came, Rajesh and Nupur Talwar were serving their life sentences in Ghaziabad's Dasna jail.
Fourteen-year-old Aarushi was found dead inside her room in the Talwars' Noida residence with her throat slit in May 2008. The needle of suspicion had initially moved towards 45-year-old Hemraj, who had gone missing but his body was recovered from the terrace of the house two days later.
As the Uttar Pradesh Police drew flak over a shoddy probe into the case which was making national headlines, the case was handed over to the CBI on a recommendation by the then chief minister Mayawati.
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