Baggio Leung and Yau Wai-ching, who deliberately misread their oaths of office, inserted expletives and draped themselves with "Hong Kong is not China" flags during a swearing-in ceremony last month, said they would appeal the decision.
"Mr Leung and Ms Yau have been disqualified from assuming and have vacated the office of a member of the Legislative Council," judge Thomas Au said in a written judgement.
Au also said the way the democratically elected pair took their oaths showed "clearly that they did not truthfully and faithfully intend to commit themselves to uphold and abide by" the city's constitution.
Leung said they would appeal the ruling.
"The judgement simply reflects that elections in Hong Kong are meaningless and the result... Can be easily overturned by the government," said Leung.
"We will go forward and appeal to the Court of Final Appeal if that is where we have to go to see justice served."
"The government has used so many small acts to suppress the courts and the courts had so much pressure and came up with such a decision," she said.
Links to the pair's biographies were no longer accessible on the Legislative Council (Legco) website Tuesday evening.
The High Court's decision was preempted by Beijing's ruling last week, which said that any oath taker who does not follow the prescribed wording of the oath, "or takes the oath in a manner which is not sincere or not solemn", should be disqualified.
"With or without (Beijing's) interpretation, the court would reach the same conclusion," Au said in the ruling.
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