"A bus hit a mine near Ansongo," a small town about 100 kilometres (60 miles) from Gao," the regional hub, a Malian military officer in the area said by phone.
"At least four civilians, including a teenage girl, were killed. There were injured as well."
A local official, reached by AFP, added: "It was jihadists who laid the bomb in order to terrorise local people, whom they accuse of providing information to the security forces."
The passengers were heading to a weekly fair in a location several dozen kilometres from Ansongo, the Gao region transport association told AFP.
Islamist extremists linked to Al-Qaeda took control of the desert north of Mali in early 2012 at the expense of Tuareg rebels, but were chased out of Sahara towns by an ongoing French-led military operation launched in January 2013.
In mid-2015, a peace accord was signed with Tuareg leaders aimed at isolating the jihadists.
But much of the region remains lawless, despite efforts by Mali's army, French soldiers and a UN force, MINUSMA.
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