Barcelona conceded twice in 68 seconds and Real Madrid were three down at half-time as La Liga's two title favourites were each stunned by surprise defeats.
Barca's 2-1 loss to relegation fighters Leganes was the greater shock but Madrid's beating was more emphatic, blown away 3-0 by Sevilla after a torrid 39 minutes at the Ramon Sanchez Pizjuan on Wednesday.
For both, it was their first defeat of the season and for both, defensive fragility was the key factor, which will be a major concern with the second round of Champions League games to come next week.
By the end, Atletico Madrid, who had cruised to victory over Huesca on Tuesday and play Real on Saturday, were the biggest winners. After a stuttering start, Diego Simeone's side will jump above Real with a win at the Santiago Bernabeu.
Sevilla can be a formidable opponent on their own patch but few expected Leganes to inflict what was only Barcelona's second league defeat in 17 months.
"We are in a very difficult moment that was unexpected," Barca coach Ernesto Valverde said.
Gerard Pique committed the decisive error, his fluffed clearance allowing Oscar Rodriguez to score Leganes' second goal in two incredible minutes at Estadio Municipal de Butarque.
Nabil El Zhar had nodded in an equaliser moments before and by the end Philippe Coutinho's sumptuous early volley was reduced to little more than a footnote.
After drawing at home to Girona on Sunday, albeit with 10 men, Valverde's selection also came under scrutiny, after he opted to rotate for the second time in three days.
Against Girona, it was Coutinho, Ivan Rakitic and Samuel Umtiti on the bench and just as they all had to be called upon in the second half at the Camp Nou, so were Luis Suarez and Jordi Alba this time.
"Of course the coach is responsible, a losing coach always feels responsible," Valverde said.
"But obviously it is easy now to talk about the rotations - in the end, matches are analysed on results - but you can't win the league with only eleven players."
"There are no excuses. We have to concede fewer goals and be more solid. Today the league isn't lost but we might miss these three points at the end of the season."
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