In a statement, Barclays said the Financial Conduct Authority (FCA) and the Prudential Regulation Authority (PRA) have "commenced investigations into Jes Staley, as to his senior manager responsibilities relating to Barclays whistleblowing programme, and Barclays Bank."
The board said it had accepted Staley's explanation that he didn't know he was not allowed to try and identify the author of a whistleblowing letter sent in 2016.
"The board has concluded, that Mr Staley honestly, but mistakenly, believed that it was permissible to identify the author of the letter," it said.
Staley said he had apologised to the Barclays board and accepted its findings.
"Our whistleblowing process is one of the most important means by which we protect our culture and values at Barclays and I certainly want to ensure that all colleagues, and others who may utilise it, understand the criticality which I attach to it," he said.
An anonymous letter was sent to board members last year raising concerns about a senior employee who had recently been recruited, including issues of a personal nature, the bank explained.
The board found out about Staley's actions early this year and instructed an external law firm, Simmons & Simmons, to investigate, as well as informing the FCA and PRA.
"I am personally very disappointed and apologetic that this situation has occurred, particularly as we strive to operate to the highest possible ethical standards," said Chairman John McFarlane.
The British bank, which was hit hard by the financial crisis, appointed Staley, a former JP Morgan investment banker, as its chief executive in 2015.
Staley's predecessor Antony Jenkins was sacked by the bank in July 2015 in the wake of the scandals.
Jenkins had been in the job since July 2012 when he replaced Bob Diamond -- who himself was forced to resign after the Libor rate-fixing scandal.
In May 2015, Barclays was hit with a USD 2.4-billion (2.2-billion-euro) fine by US and British regulators for manipulation of foreign exchange trading. Five other global banks have been fined over the affair.
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