Thousands of people cheered and waved flags as they watched the vessel, especially renamed COSCO Shipping Panama, inaugurate the widening of the century-old waterway, which has been fitted with a new shipping lane and locks.
"This is a great day, a day of national unity and a day for Panama," President Juan Carlos Varela said in a speech.
"This is the route that unites the world," he said.
The Chinese ship entered from the Atlantic and was to take hours to make its way to the Pacific side of the country, where a ceremony attended by several heads of state and foreign dignitaries was to take place.
The United States and China are the two most frequent canal users. Its expansion is expected to greatly benefit commercial traffic between North America and Asia.
The expansion work carried out since 2007 - and finished two years late at a cost of at least USD 5.5 billion - allows a new generation of much larger ships, known as Neopanamax class vessels, to ply the canal.
The expansion will also allow Panama to lure massive liquified natural gas (LNG) tankers for the first time.
They represent a lucrative segment of the shipping market whose importance has grown with the development of US exports of natural gas from shale, most of which head to Japan and South Korea.
Varela said yesterday the first LNG vessel is scheduled to cross the canal next month. He predicts that many more will follow.
However, that goal might still be a decade or more away, according to officials from the Panama Canal Authority, the autonomous government agency that runs the waterway.
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