Driver Gul Muhammad could not record his statement as he had received 90 per cent burn injuries, a Punjab government spokesman said.
"The death toll in Bahwalpur oil tanker tragedy has risen to 165. The driver of the tanker also succumbed to his injuries," he said.
So far, 125 people have been laid to rest after establishing their identity through DNA test, he said, adding that the identification of others was underway.
Some 151 people lost their lives while collecting spilt fuel as the oil tanker caught fire and exploded. Over 140 others suffered injuries in one of the deadliest accidents in the country.
Video images of the people gathered to collect petrol from the overturned oil tanker emerged showing young and old people filling their bikes with the spilled oil and collecting it in bottles and cans.
According to a preliminary report, the tragedy might have been averted had the motorway police reached there on time and cordoned off the area.
Prime Minister Nawaz Sharif said "illiteracy and poverty" is a reason behind this incident.
More than 40 injured are still battling for their lives in burn centres of Multan and Lahore.
A video before the incident also emerged on social media showing that a man was smoking a cigarette and he inadvertently threw its butt.
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