The film delves into the controversy surrounding Dr Mukherjee's feat, which had led the scientist to commit suicide.
Directed by social activist Rajib Sarkar, the documentary "Blood stains never fade" was produced by "India's Smile".
It was a sequel to "Effect of Indian Crab Syndrome" which was screened last year at the Kolkata International Film Festival.
The director said that 35 years ago the scientist had committed suicide after his stellar achievement was rejected, but now he had been recognised as a genius ahead of his time.
The documentary quoted the state's former health minister and CPI-M leader Dr Surjya Kanta Mishra as saying that scientists of his time could not understand the importance of his work.
"I do not believe that somebody conspired against him. It happened due to their ignorance," he said.
Dr Mukherjee has also been the subject of eminent director Tapan Sinha's Hindi film 'Ek Doctor Ki Maut' (Death of a doctor).
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