Hacene Ould Khalil, who went by the nom de guerre Jouleibib, was killed last week in the Tessalit region, said a regional security source who told AFP the Mauritanian national was Belmokhtar's "genuine right-hand man".
Jouleibib was the deputy commander of the Signatories in Blood, an armed unit founded by Belmokhtar last year after the one-eyed Algerian broke away from Al-Qaeda in the Islamic Maghreb.
France's military spokesman in Paris would not comment on the operation but a second regional security source in Mali confirmed the information.
As well as being a fighter, Jouleibib also acted as a spokesman for the Signatories in Blood.
"He was responsible for the management of the group. It is truly a blow to Belmokhtar," the source added.
France's chief of defence staff in Paris said last Thursday that French forces had "neutralised" several Al-Qaeda members in northern Mali.
The special operation overnight in the desert also led to the seizure of weapons, Admiral Edouard Guillaud said on Europe 1 radio.
The Signatories in Blood allegedly masterminded a siege in January of an Algerian gas plant in which 38 hostages died.
Branded "The Uncatchable", Belmokhtar is also thought to have been behind twin car bombings in Niger in May that left at least 20 people dead.
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