G-7 diplomats sitting down for talks in the centuries-old Ducal Palace in Lucca, Italy, hope to use outrage over the attack and wide international support for the United States' retaliatory missile strikes to push Russia to abandon Assad and join a new peace effort for Syria.
Members of the group also hope to gain a sense from US Secretary of State Rex Tillerson of President Donald Trump's next steps and foreign-policy goals.
He said Russia had a choice: to continue backing the "toxic" Assad regime, "or to work with the rest of the world to find a solution for Syria, a political solution".
Last week's nerve gas attack in the rebel-held town of Khan Sheikhoun, which killed more than 80 people, stirred Trump, who was previously cool to the idea of US intervention, to strike for the first time at Assad's forces. US warships fired 59 cruise missiles at the Syrian air base from which the US believes the attack was launched.
In a gesture weighted with symbolism, Tillerson visited the site of a World War II-era Nazi massacre in central Italy today. He said the United States was rededicating itself to hold to account "any and all" who commit crimes against innocent people.
Tillerson accompanied Alfano to Santa'Anna di Stazzema, where 560 civilians, including some 130 children, were killed in 1944.
Ahead of the full meeting, Tillerson held bilateral talks with G-7 counterparts including Britain's Johnson, French Foreign Minister Jean-Marc Ayrault and Japanese Foreign Minister Fumio Kishida.
Kishida said that "Japan supports the US commitment in trying to take responsibility to prevent spread and use of chemical weapons and we confirmed Japan and the US will continue to work together (in that effort)".
The G-7 meeting comes as the United States is sending a Navy carrier strike group towards the Korean Peninsula in a show of strength following North Korea's persistent ballistic missile tests.
It is also taking place amid an ongoing terror threat that was underscored by the Palm Sunday bombing of Coptic churches in Egypt claimed by the Islamic State group, and another truck attack on European soil, this time in Stockholm, on Friday.
Syria, though, topped the agenda.
Russia planned to put forward a proposal today for an independent and impartial investigation of the attack, a spokesman for German Foreign Minister Sigmar Gabriel said, calling it "a good and important sign".
Iranian Foreign Minister Mohammad Javad Zarif, whose government is another backer of Assad's, also called for an independent inquiry under UN auspices when he spoke today to Alfano, Italy's foreign ministry said in a statement.
The United States is fighting Islamic State group militants in Syria, but had previously avoided striking government forces, largely out of concern about being pulled into a military conflict with Russia, whose relations with the West have been on a downward spiral for several years.
The flipside of the talk about sanctions from Johnson and other diplomats is an implicit promise that Moscow could be allowed to rejoin the G-8, if it drops its support for Assad.
"I think the Russians need a way out and a way forward," Johnson said.
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