The 44-year-old musician made a surprise appearance at Anita Morrissey-Booth and Alex Morrissey's ceremony at Knowsley Hall last week, reported Digital Spy.
Barlow performed Take That's "A Million Love Songs" for the newlyweds.
The musician previously promised to sing at his three fans' weddings in 2015.
In a series of posts on social media back in January, Barlow said that the recipients must be huge fans and stalk him on Twitter.
He previously appeared at Danielle Jones's reception in Bracknell, Berkshire in April.
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