The head of the national police force, Nery Ramos, said police stations across the country were on alert looking out for Marixa Lemus, 45, following her jail break from a military penitentiary north of Guatemala City.
"An alert has gone out nationally and operations are underway to locate her," he said.
Nicknamed "The Boss" ("La Patrona" in Spanish), Lemus started her lengthy sentence in March 2015 after being convicted of heading up the gang that carried out contract killings and kidnappings.
Lemus had tried to escape once before, from a women's prison in the capital in May last year, but police caught her hiding in woodland near the penitentiary.
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