Mohamed Zaouari, a 49-year-old engineer, was murdered at the wheel of his car outside his house in Tunisia's second city, Sfax, on Thursday.
Al-Qassam Martyrs Brigade, the armed wing of Hamas that controls the Gaza Strip, told AFP today that drone expert Zaouari was killed by "Zionist treachery", referring to Israel.
It said Zaouari had worked for the "resistance" for 10 years, and announced a day of mourning.
"Zaouari's assassination in Tunisia is aggression against the resistance, and Al-Qassam Brigades and the enemy should know that the blood of the leaders will not be wasted and lost in vain," it said in a separate statement.
Authorities in Tunisia have said at least five suspects have been detained in Sfax, Tunis and Djerba, and four vehicles seized along with two guns equipped with silencers.
Private radio station Mosaique FM reported that Zaouari's body was riddled with 20 bullets.
Israel has ignored the Hamas allegation, despite its record of assassinating members of militant groups abroad, including in Tunisia.
In 1988, the head of the Palestine Liberation Organisation military wing Khalil al-Wazir, better known as Abu Jihad, was killed by Israeli commandos in Tunisia with another senior PLO figure, Saad Sayil.
Mossad is also believed to have been behind the 2010 murder of top Hamas militant Mahmud al-Mabhuh in a Dubai hotel.
And Imad Mughniyeh, a top operative with Lebanon's Shiite group Hezbollah, was killed in Damascus in a 2008 bombing that drew immediate threats of retaliation against Israel.
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