Cooley, who was the founder of Texas Heart Institute, pioneered many techniques still used in cardiovascular surgery.
He and his teams also performed more than 118,000 open-heart operations.
Former President George H W Bush said in a statement: Barbara and I join the thousands of Houstonians, Americans, and admirers around the world who today are mourning the loss of a true giant, Denton Cooley.
"Denton's pioneering contributions to medicine are, of course, legend. But he also was a lifelong and leading citizen of Houston. All of us who call Houston home will always feel blessed to live in the city where Denton founded the Texas Heart Institute, making our hometown the global centre of cardiovascular research and technology," he said.
His wife,Louise Goldsborough Thomas Cooley,died just a few weeks earlieron October 21 at the age of 92.
His family shared memories of a man who pioneered heart transplantation, developed an artificial heart and founded the Texas Heart Institute.
"He made sure he had time for each one of us, all of my children, all of their children, all of our grandchildren," Susan Cooley said.
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