The advances in the northern Aleppo province brought the militants to within few kilometers from the border town of Azaz, where rebels hold an enclave that is hosting tens of thousands of internally displaced civilians.
In recent months, Syrian rebel factions in Azaz, and its border crossing of Bab al-Salama, have separately come under fire from the extremist IS group, pro-government forces and the predominantly Kurdish Syrian Democratic Forces.
Both Azaz and the Bab al-Salama crossing have been a lifeline for the opposition since the town fell into rebel hands in 2012, up until recently.
The IS news agency, Aamaq, also reported the advance, saying the Islamic State group seized six villages from rebels.
The humanitarian medical organisation Doctors Without Borders said its team is currently evacuating patients and staff from the Al Salama hospital, which it runs in Azaz, after the frontline shifted to within three kilometers (2 miles) from the facility.
"MSF has had to evacuate most patients and staff from our hospital as front lines have come too close," said Pablo Marco, MSF operations manager for the Middle East.
"We are terribly concerned about the fate of our hospital and our patients, and about the estimated 100,000 people trapped between the Turkish border and active front lines. "There is nowhere for people to flee to as the fighting gets closer," he said.
The predominantly Kurdish Syrian Democratic Forces (SDF), who are fighting for their autonomy in the multilayered conflict, also gained ground against the rebels.
That left the rebels in Aleppo with just one narrow corridor to the outside world, through Idlib province. Those in Azaz are now squeezed between IS to the east and the SDF to the west and south, while Turkey tightly restricts the flow of goods and people through the border.
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