Palestinians will be barred from entering from the West Bank and Gaza Strip as the Sukkot festival wraps up with the Simchat Torah holiday, which begins at sundown today and ends the next evening.
The closure, a frequent measure for major Jewish holidays, will remain in place throughout Sunday and Monday, with only emergency cases allowed through, the Israeli army said.
Israeli police also announced they had arrested 19 Palestinians in various areas of annexed east Jerusalem in connection with recent clashes with security forces.
The week-long Sukkot festival is the last of three successive Jewish holidays that have led to tensions with the Palestinians in the past.
Earlier in October, Jews marked Yom Kippur and Rosh Hashanah, or New Year.
A Palestinian gun attack in Jerusalem on October 9 that killed two people raised fears of further violence and Israel has boosted security.
Last year's holiday period led to clashes and marked the start of an upsurge in Palestinian gun, knife and car-ramming attacks.
The compound is holy to both Muslims and Jews, who refer to it as the Temple Mount.
The site is central to the Israeli-Palestinian conflict, with Palestinians fearing Israel may one day seek to assert further control over it.
Violence since October 2015 has killed 235 Palestinians, 36 Israelis, two Americans, one Jordanian, an Eritrean and a Sudanese national, according to an AFP count.
Most of the Palestinians killed were carrying out knife, gun or car-ramming attacks, according to Israeli authorities.
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